A preliminary investigation of in vitro anti-thrombotic and anti-platelet activity of Pinus Gerardiana

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Atta-ur Rehman Sara Naz Muhammad Zaman Syed Saeed-ul-Hassan Javed Iqbal Awais Ali Zaidi

Abstract

Introduction: Hemostasis is a process which preserves the stability of a closed and high-pressure circulatory system after any vascular injury. Circulating platelets are recruited to the site of injury, where they develop a major component of the developing thrombus, blood clotting, started by tissue factor, concludes in the generation of thrombin and fibrin. Thrombosis is a serious event in the arterial diseases and a major cause in the development of myocardial infarction, stroke and venous thrombo-embolism which justify prominent morbidity and mortality rate. The knowledge of molecular and cellular mechanism of the formation of thrombus has developed considerably in the recent studies by using different in-vitro and in-vivo models of diseases. P. gerardiana nut oil has been reported to possess anti-bacterial, anti-fungal, anti-viral, anti-septic, anti-neuralgic, diuretic, expectorant, hypertensive properties. However, hardly, any data is available regarding effects of nut oil on platelet function. In this study, fibrinolytic activity and effect on platelet aggregation were investigated. Method: P. gerardiana nut oil was extracted by using n-Hexane and then concentrated by rotary evaporator. Anti-thrombotic and fibrinolytic activities were evaluated on blood clot formation. Effects on platelet aggregation of the oil were determined based on collagen or epinephrine induced platelet aggregation. Results: P. gerardiana caused blood clot lysis in-vitro. P. gerardiana nut oil inhibited collagen dependent platelet aggregation while accelerated the epinephrine dependent platelet aggregation. In vitro whole blood coagulation was also reduced. In vivo P. gerardiana nut oil has no significant effect on blood cell indices. Conclusion: P. gerardiana nuts oil can be an effective therapy for the treatment of cardiovascular disorders and thromboembolism.

References

Al-Mamun, M.R., Amrin, N., Begum, J., and Mazid, M.A. (2012). Thrombolytic activity of some spices and plants available in Bangladesh. The Thai Journal of Pharmaceutical Sciences 36, 72-77.
Amr, A.R., and Abeer, E. (2011). Hypolipideimic and hypocholestermic effect of pine nuts in rats fed high fat, cholesterol-diet. World Applied Sciences Journal 15, 1667-1677.
Bartimoccia, S., Nocella, C., Pastori, D., Pignatelli, P., and Carnevale, R. (2015). Platelet Oxidative Stress and Antioxidant Nutrients. Journal of Vascular Medicine & Surgery 2014.
Baumgartner, H.R., and Haudenschild, C. (1972). Adhesion of platelets to subendothelium. Annals of the New York Academy of Sciences 201, 22-36.
Biggs, R. (1972). Human blood coagulation, haemostasis and thrombosis.
Brufau, G., Boatella, J., and Rafecas, M. (2006). Nuts: source of energy and macronutrients. British Journal of Nutrition 96, S24-S28.
Campbell, N.A., Reece, J.B., Urry, L.A., Cain, M.L., Wasserman, S.A., Minorsky, P.V., and Jackson, R.B. (2008). AP Edition Biology (Benjamin/Cummings).
Choi, J.W. (2002). Incidence of nonresponsiveness to epinephrine in platelets from healthy humans. Acta haematologica 108, 106-108.
Cowan, D.H., Robertson, A.L., Shook, P., and Giroski, P. (1981). Platelet adherence to collagen: role of plasma, ADP, and divalent cations. British journal of haematology 47, 257-267.
Destaillats, F.d.r., Cruz-Hernandez, C., Giuffrida, F., and Dionisi, F. (2010). Identification of the botanical origin of pine nuts found in food products by gas− liquid chromatography analysis of fatty acid profile. Journal of agricultural and food chemistry 58, 2082-2087.
Elliott, W.H., Elliott, D.C., Jefferson, J.R., and Wheldrake, J. (1997). Biochemistry and molecular biology (Oxford University Press Oxford).
Furie, B., and Furie, B.C. (2008). Mechanisms of thrombus formation. New England Journal of Medicine 359, 938-949.
Hussain, J., Jamila, N., Gilani, S.A., Abbas, G., and Ahmed, S. (2009). Platelet aggregation, antiglycation, cytotoxic, phytotoxic and antimicrobial activities of extracts of Nepeta juncea. African Journal of Biotechnology 8.
Lanza, F., Beretz, A., Stierle, A., Hanau, D., Kubina, M., and Cazenave, J.-P. (1988). Epinephrine potentiates human platelet activation but is not an aggregating agent. American Journal of Physiology-Heart and Circulatory Physiology 255, H1276-H1288.
Lawlees, J. (1992). The encyclopaedia of essential oils (Element Books Limited: Boston).
Li, H., Huang, W., Wen, Y., Gong, G., Zhao, Q., and Yu, G. (2010). Anti-thrombotic activity and chemical characterization of steroidal saponins from Dioscorea zingiberensis CH Wright. Fitoterapia 81, 1147-1156.
Mekhfi, H., ElHaouari, M., Bnouham, M., Aziz, M., Ziyyat, A., and Legssyer, A. (2006). Effects of extracts and tannins from Arbutus unedo leaves on rat platelet aggregation. Phytotherapy research 20, 135-139.
Michelson, A.D., Frelinger, A.L., and Furman, M.I. (2006). Current options in platelet function testing. The American journal of cardiology 98, S4-S10.
Morton, L.F., Peachey, A.R., and Barnes, M.J. (1989). Platelet-reactive sites in collagens type I and type III. Evidence for separate adhesion and aggregatory sites. Biochemical Journal 258, 157-163.
Nakano, T., Terawaki, A., and Arita, H. (1986). Measurement of Thromboxane A2 Elevation of Ionized Calcium in Collagen-Stimulated Platelets with the Photoprotein, Aequorin. Journal of biochemistry 99, 1285-1288.
Pierre, S., Crosbie, L., and Duttaroy, A.K. (2005). Inhibitory effect of aqueous extracts of some herbs on human platelet aggregation in vitro. Platelets 16, 469-473.
Pollock, W.K., Rink, T.J., and Irvine, R.F. (1986). Liberation of [3H] arachidonic acid and changes in cytosolic free calcium in fura-2-loaded human platelets stimulated by ionomycin and collagen. Biochemical Journal 235, 869-877.
Poole, A.W., and Watson, S.P. (1995). Regulation of cytosolic calcium by collagen in single human platelets. British journal of pharmacology 115, 101-106.
Porth, C. (2005). Pathophysiology: Concepts of altered health states, Vol 1 (Lippincott Williams & Wilkins).
Rittenhouse, S., and Allen, C. (1982). Synergistic activation by collagen and 15-hydroxy-9 alpha, 11 alpha-peroxidoprosta-5, 13-dienoic acid (PGH2) of phosphatidylinositol metabolism and arachidonic acid release in human platelets. Journal of Clinical Investigation 70, 1216.
Roth, G.J., and Majerus, P.W. (1975). The mechanism of the effect of aspirin on human platelets. I. Acetylation of a particulate fraction protein. Journal of Clinical Investigation 56, 624-632.
Sagrero‐Nieves, L. (1992). Fatty acid composition of Mexican pine nut (Pinus cembroides) oil from three seed coat phenotypes. Journal of the Science of Food and Agriculture 59, 413-414.
Sànchez-Lamar, A., Fiore, M., Cundari, E., Ricordy, R., Cozzi, R., and De Salvia, R. (1999). Phyllanthus orbicularis aqueous extract: cytotoxic, genotoxic, and antimutagenic effects in the CHO cell line. Toxicology and applied pharmacology 161, 231-239.
Santoro, S.A., Walsh, J., Staatz, W., and Baranski, K. (1991). Distinct determinants on collagen support alpha 2 beta 1 integrin-mediated platelet adhesion and platelet activation. Cell regulation 2, 905-913.
Savage, G.P. (2001). Chemical composition of walnuts (Juglans regia L.) grown in New Zealand. Plant Foods for Human Nutrition 56, 75-82.
Sharma, A., Goyal, R., and Sharma, L. (2016). Potential biological efficacy of Pinus plant species against oxidative, inflammatory and microbial disorders. BMC Complementary and Alternative Medicine 16, 35.
Strukova, S. (2001). Thrombin as a regulator of inflammation and reparative processes in tissues. Biochemistry (Moscow) 66, 8-18.
Umesh, M., Sanjeevkumar, C., Hanumantappa, B., and Ramesh, L. (2014). Evaluation of in vitro anti-thrombolytic activity and cytotoxicity potential of Typha angustifolia L leaves extracts. Int J Pharm Pharm Sci 6, 81-85.
Yavasoglu, I., Acar, B., Kadikoylu, G., and Bolaman, Z. (2010). Platelet aggregation tests are affected in pseudothrombocytopenia. Laboratory Medicine 41, 483-485.

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REHMAN, Atta-ur et al. A preliminary investigation of in vitro anti-thrombotic and anti-platelet activity of Pinus Gerardiana. Biomedical Research and Therapy, [S.l.], v. 4, n. 1, p. 1098-1109, jan. 2017. ISSN 2198-4093. Available at: <http://www.bmrat.org/index.php/BMRAT/article/view/145>. Date accessed: 23 oct. 2017. doi: https://doi.org/10.15419/bmrat.v4i1.145.
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Research articles