Comparative treatment efficiency of adipose and bone marrow derived allogenic mesenchymal stem cell transplantation in mouse models of liver fibrosis

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Nam Hai Nguyen Trinh Van Le Huy Quang Do Dat Quoc Ngo Huy Minh Le Nhung Hai Truong

Abstract

Background: The application of mesenchymal stem cell (MSC) therapy in liver fibrosis treatment has been increasingly investigated in recent years. MSCs obtained from a variety of sources (e.g. bone marrow, umbilical cord blood and adipose tissue) have been studied and have achieved remarkable results. In this study, we compared the effects of adipose-derived mesenchymal stem cells (AD-MSC) transplantation with bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation in a mouse model of liver fibrosis, induced by carbon tetrachloride (CCl4).

Methods: Eight-week old mice were treated with CCl4 for 11 weeks to induce liver fibrosis then 5x105 cells were transplanted into mice via the tail vein.

Results: After 21 days of transplantation, the results showed that the stem cell treated groups ameliorated better than the placebo group. MSC treated groups showed reduced AST and ALT levels, down-regulated expression of extracellular matrix (ECM) genes, and improved liver histopathology. Both sources of MSCs (bone marrow and adipose tissue) were effective in the mouse model of liver fibrosis.

Conclusion: Our results also indicated that AD-MSC transplantation in mice accelerated liver regeneration better than BM-MSC transplantation.

References

Abdel Aziz, M.T., Atta, H.M., Mahfouz, S., Fouad, H.H., Roshdy, N.K., Ahmed, H.H., Rashed, L.A., Sabry, D., Hassouna, A.A., and Hasan, N.M. (2007). Therapeutic potential of bone marrow-derived mesenchymal stem cells on experimental liver fibrosis. Clinical biochemistry 40, 893-899.
Abdel Aziz, M.T., El Asmar, M.F., Mostafa, S., Salama, H., Atta, H.M., Mahfouz, S., Roshdy, N.K., Rashed, L.A., Sabry, D., Hasan, N., et al. (2010). Reversal of Hepatic Fibrosis by Human CD34(+) Stem/Progenitor Cell Transplantation in Rats. International Journal of Stem Cells 3, 161-174.
Ali, G., and Masoud, M.S. (2012). Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl(4)-induced fibrotic liver. Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association 18, 263-267.
Baligar, P., Mukherjee, S., Kochat, V., Rastogi, A., and Mukhopadhyay, A. (2016). Molecular and Cellular Functions Distinguish Superior Therapeutic Efficiency of Bone Marrow CD45 Cells Over Mesenchymal Stem Cells in Liver Cirrhosis. Stem cells 34, 135-147.
Berardis, S., Dwisthi Sattwika, P., Najimi, M., and Sokal, E.M. (2015). Use of mesenchymal stem cells to treat liver fibrosis: current situation and future prospects. World journal of gastroenterology 21, 742-758.
Bifari, F., Lisi, V., Mimiola, E., Pasini, A., and Krampera, M. (2008). Immune Modulation by Mesenchymal Stem Cells. Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie 35, 194-204.
Bigot, N., Mouche, A., Preti, M., Loisel, S., Renoud, M.L., Le Guevel, R., Sensebe, L., Tarte, K., and Pedeux, R. (2015). Hypoxia Differentially Modulates the Genomic Stability of Clinical-Grade ADSCs and BM-MSCs in Long-Term Culture. Stem cells 33, 3608-3620.
Choudhery, M.S., Badowski, M., Muise, A., and Harris, D.T. (2013). Comparison of human mesenchymal stem cells derived from adipose and cord tissue. Cytotherapy 15, 330-343.
Eirin, A., Zhu, X.Y., Ferguson, C.M., Riester, S.M., van Wijnen, A.J., Lerman, A., and Lerman, L.O. (2015). Intra-renal delivery of mesenchymal stem cells attenuates myocardial injury after reversal of hypertension in porcine renovascular disease. Stem cell research & therapy 6, 7.
Francois, S., Mouiseddine, M., Allenet-Lepage, B., Voswinkel, J., Douay, L., Benderitter, M., and Chapel, A. (2013). Human mesenchymal stem cells provide protection against radiation-induced liver injury by antioxidative process, vasculature protection, hepatocyte differentiation, and trophic effects. BioMed research international 2013, 151679.
Harn, H.J., Lin, S.Z., Hung, S.H., Subeq, Y.M., Li, Y.S., Syu, W.S., Ding, D.C., Lee, R.P., Hsieh, D.K., Lin, P.C., et al. (2012). Adipose-derived stem cells can abrogate chemical-induced liver fibrosis and facilitate recovery of liver function. Cell transplantation 21, 2753-2764.
Kassebaum, N.J., Arora, M., Barber, R.M., Bhutta, Z.A., Brown, J., Carter, A., Casey, D.C., Charlson, F.J., Coates, M.M., Coggeshall, M., et al. (2016). Global, regional, and national disability-adjusted life-years (DALYs) for 315 diseases and injuries and healthy life expectancy (HALE), 1990–2015: a systematic analysis for the Global Burden of Disease Study 2015. The Lancet 388, 1603-1658.
Koppula, P.R., Chelluri, L.K., Polisetti, N., and Vemuganti, G.K. (2009). Histocompatibility testing of cultivated human bone marrow stromal cells - a promising step towards pre-clinical screening for allogeneic stem cell therapy. Cellular immunology 259, 61-65.
Matsuda-Hashii, Y., Takai, K., Ohta, H., Fujisaki, H., Tokimasa, S., Osugi, Y., Ozono, K., Matsumoto, K., Nakamura, T., and Hara, J. (2004). Hepatocyte growth factor plays roles in the induction and autocrine maintenance of bone marrow stromal cell IL-11, SDF-1 alpha, and stem cell factor. Experimental hematology 32, 955-961.
Mederacke, I. (2013). Liver fibrosis - mouse models and relevance in human liver diseases. Zeitschrift fur Gastroenterologie 51, 55-62.
Mortality, G.B.D., and Causes of Death, C. (2015). Global, regional, and national age-sex specific all-cause and cause-specific mortality for 240 causes of death, 1990-2013: a systematic analysis for the Global Burden of Disease Study 2013. Lancet 385, 117-171.
Nicolay, N.H., Lopez Perez, R., Debus, J., and Huber, P.E. (2015). Mesenchymal stem cells - A new hope for radiotherapy-induced tissue damage? Cancer letters 366, 133-140.
Parekkadan, B., van Poll, D., Megeed, Z., Kobayashi, N., Tilles, A.W., Berthiaume, F., and Yarmush, M.L. (2007). Immunomodulation of activated hepatic stellate cells by mesenchymal stem cells. Biochemical and biophysical research communications 363, 247-252.
Reinisch, A., Etchart, N., Thomas, D., Hofmann, N.A., Fruehwirth, M., Sinha, S., Chan, C.K., Senarath-Yapa, K., Seo, E.Y., Wearda, T., et al. (2015). Epigenetic and in vivo comparison of diverse MSC sources reveals an endochondral signature for human hematopoietic niche formation. Blood 125, 249-260.
Schuppan, D., and Afdhal, N.H. (2008). Liver cirrhosis. Lancet 371, 838-851.
Seki, A., Sakai, Y., Komura, T., Nasti, A., Yoshida, K., Higashimoto, M., Honda, M., Usui, S., Takamura, M., Takamura, T., et al. (2013). Adipose tissue-derived stem cells as a regenerative therapy for a mouse steatohepatitis-induced cirrhosis model. Hepatology 58, 1133-1142.
Yuan, S., Jiang, T., Zheng, R., Sun, L., Cao, G., and Zhang, Y. (2014). Effect of bone marrow mesenchymal stem cell transplantation on acute hepatic failure in rats. Experimental and therapeutic medicine 8, 1150-1158.
Zhang, Y., Chen, X.M., and Sun, D.L. (2014). Effects of coencapsulation of hepatocytes with adipose-derived stem cells in the treatment of rats with acute-on-chronic liver failure. The International journal of artificial organs 37, 133-141.
Zhao, D.C., Lei, J.X., Chen, R., Yu, W.H., Zhang, X.M., Li, S.N., and Xiang, P. (2005). Bone marrow-derived mesenchymal stem cells protect against experimental liver fibrosis in rats. World journal of gastroenterology 11, 3431-3440.

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How to Cite
NGUYEN, Nam Hai et al. Comparative treatment efficiency of adipose and bone marrow derived allogenic mesenchymal stem cell transplantation in mouse models of liver fibrosis. Biomedical Research and Therapy, [S.l.], v. 4, n. 06, p. 1374-1387, june 2017. ISSN 2198-4093. Available at: <http://www.bmrat.org/index.php/BMRAT/article/view/179>. Date accessed: 23 oct. 2017. doi: https://doi.org/10.15419/bmrat.v4i06.179.
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Research articles