ID3007 Mechanisms underlying the anticancer activities of the selected phytochemicals and their therapeutic implication in AGS gastric cancer cells

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Somi Kim Cho

Abstract

The cysteine-rich angiogenic inducer 61 (CYR61), an extracellular matrix-associated protein, is involved in survival, tumorigenesis, and drug resistance. There is an increasing demand for developing agents that target CYR61. Hence, we study the effects of flavones against CYR61-overexpressing human gastric adenocarcinoma AGS (AGS-cyr61) cells. Among the various flavones, quercetin had lowest IC50 value and reduced the viability of AGS-cyr61 cells even greater than that of AGS cells. Quercetin (1) down-regulates CYR61 and concomitantly decreases in the levels of MRP1 (multidrug resistance-associated protein 1) and nuclear factor NF-kappa B (κB) p65 subunit, (2) reverses multidrug resistance, and (3) inhibits colony formation in AGS-cyr61 cells. AGS-cyr61 cells treated with quercetin at sub IC50 over a range of 5-FU or ADR concentrations manifested strong synergistic effects with these two drugs. Our results demonstrate that CYR61 is a potential regulator of ABC transporters and quercetin can be the novel agent that improves the efficacy of anticancer drugs by down-regulating CYR61 and ABC transporters.

Histone deacetylase 6 (HDAC6) is a unique cytoplasmic enzyme which contributes to malignant progression in various cancer. Such effect on cancer brings more interest on developing HDAC6 inhibitors. Here, we found that compound D inhibits HDAC6 activity, increases acetylated α-tubulin, reduces the level of β-catenin, and suppresses cell proliferation. Increase of α-tubulin acetylation by compound D resulted in tubulin polymerization, and consequently, induced aberrant mitosis. Moreover, DTBP elicits different effects depending on different concentrations.

 

Treatment with high concentrations of compound D induces cell death by mitotic catastrophe, whereas low concentration of compound D induces senescence with upregulation of p21 and Rb, and increase in the phosphorylation of mTOR and the β-galactosidase activity. Therefore, compound D can also be considered as a promising new candidate for anti-cancer drug development.

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How to Cite
CHO, Somi Kim. ID3007 Mechanisms underlying the anticancer activities of the selected phytochemicals and their therapeutic implication in AGS gastric cancer cells. Biomedical Research and Therapy, [S.l.], v. 4, n. S, p. S19-S20, aug. 2017. ISSN 2198-4093. Available at: <http://www.bmrat.org/index.php/BMRAT/article/view/225>. Date accessed: 12 dec. 2017. doi: https://doi.org/10.15419/bmrat.v4iS.225.
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Oral Abstracts