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Abstract

Endothelial progenitor cells (EPCs) play an important role in angiogenesis. However, they exist in limited numbers in the human body. This study was aimed to produce EPCs, for autologous transplantation, using direct reprogramming of skin fibroblasts under GMP-compliant conditions. Fibroblasts were collected and cultured from the skin in DMEM/F12 medium supplemented with 5% activated platelet-rich plasma and 1% antibiotic-antimycotic solution. They were then transfected with mRNA ETV2 and incubated in culture medium under hypoxia (5% oxygen) for 14 d. Phenotype analysis of transfected cells confirmed that single-factor ETV2 transfection successfully reprogrammed dermal fibroblasts into functional EPCs. Our results showed that ETV2 mRNA combined with hypoxia can give rise to functional EPCs. The cells exhibited functional phenotypes similar to endothelial cells derived from umbilical cord vein; they expressed CD31 and VEGFR2, and formed capillary-like structures in vitro. Moreover, these EPCs could significantly improve hindlimb ischemia in mouse models. Although the direct conversion efficacy was low (3.12 ± 0.98%), altogether our study demonstrates that functional EPCs can be produced from fibroblasts and can be used in clinical applications.



Author's Affiliation
  • Phuc Van Pham

    Google Scholar Pubmed

  • Ngoc Bich Vu

    Google Scholar Pubmed

  • Thuy Thi-Thanh Dao

    Google Scholar Pubmed

  • Ha Thi-Ngan Le

    Google Scholar Pubmed

  • Lan Thi Phi

    Google Scholar Pubmed

  • Ngoc Kim Phan

    Google Scholar Pubmed


 Copyright Info

Creative Commons License

Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 How to Cite
Pham, P., Vu, N., Dao, T., Le, H., Phi, L., & Phan, N. (2017). ID: 1016 Production of endothelial progenitor cells from skin fibroblasts by direct reprogramming for clinical usages. Biomedical Research and Therapy, 4(S), S 91. https://doi.org/https://doi.org/10.15419/bmrat.v4iS.294

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