Observation of TNF-α, IL-10 and HB-EGF gene expression by peripheral blood CD14+ mononuclear cells: a case of guttate psoriatic patient

Main Article Content

Viet Hong Nguyen Hoa Cuc Pham

Abstract

Introduction: Guttate is a type of psoriasis in which patients are sensitive to Streptococcus pneumoniae throughout innate immune responses. During the inflammation, tumour necrosis factor alpha (TNF-α), a well-known pro-inflammatory cytokine, is expressed; meanwhile interleukin 10 (IL-10) and heparin-binding EGF-like growth factor (HB-EGF), which are capable of inhibiting transcription of the TNF-α gene, are also prominent. Furthermore, HB-EGF only impacts fibroblasts and keratinocytes which promote psoriatic lesions. In this study, we looked for differences of TNF-α, IL-10 and HB-EGF expression between a psoriatic patient and a non-psoriatic relative.

Methods: To achieve our target, peripheral blood mononuclear cells (PBMCs) expressing LPS receptors or CD14 (CD14+ cells) derived from a guttate patient, and the donor’s father (without psoriatic symptoms), were activated for 7 days by a lysate of Streptococcus pneumoniae for 24 hours before being harvested.

Results: Results showed detectable mRNAs of TNF-α, IL-10 and HB-EGF from isolated CD14+ cells of guttate patient were more intensive expression than the non-psoriatic one at 24 hours after engaging the bacterial components. In addition, transcription of HB-EGF gene from the guttate patient was maintained over 168 hours, while its mRNA level from the non-psoriatic volunteer was only expressed within 24 hours.

Conclusion: Finally, in initial results of inflammatory effects between strains, the Streptococcal lysate was seen to have stronger immune responses than the Staphylococcal lysate on the immune cells of the guttate psoriasis.

 

Peer Review Details

  • Peer review method: Single-Blind (Peer-reviewers: 02) Peer-review policy
  • Plagiarism software screening?: Yes
  • Date of Original Submission: 10 October 2017
  • Date accepted: 18 November 2017
  • Peer reviewers approved by: Dr. Lili Hami
  • Editor who approved publication: Dr. Phuc Van Pham

 

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How to Cite
NGUYEN, Viet Hong; PHAM, Hoa Cuc. Observation of TNF-α, IL-10 and HB-EGF gene expression by peripheral blood CD14+ mononuclear cells: a case of guttate psoriatic patient. Biomedical Research and Therapy, [S.l.], v. 4, n. 11, p. 1733-1748, nov. 2017. ISSN 2198-4093. Available at: <http://www.bmrat.org/index.php/BMRAT/article/view/378>. Date accessed: 14 dec. 2017. doi: https://doi.org/10.15419/bmrat.v4i11.378.
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Research articles