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Abstract

Introduction: The purpose of this study was to detect ybtS, entB, mrkD, magA, kfu, iutA, rmpA and K2 genes in extended-spectrum beta-lactamase (ESBL) - and non-ESBL producing Klebsiella pneumoniae.


Methods: To this end, 70 K. pneumoniae isolates were selected from hospitals of Kurdistan Province, Iran. The ESBL phenotype was conducted utilizing the disc diffusion technique in accordance with CLSI procedures. Detection of virulence factor genes was performed by the PCR in the ESBL and non-ESBL isolates.


Results: Sixty-two (88.6%) isolates of K. pneumoniae were ESBL producers. Further, entB had the most frequency in all the isolates. There were no significant differences between ESBL production and the presence of ybt S, entB, mrkD, magA, kfu, iutA, rmpA and K2 genes and the presence of these genes and variables such as presence of sex, clinical specimen type, and hvKP phenotype among the ESBL and non-ESBL K. pneumoniae isolates.


Conclusion: In conclusion, in other studies, K. pneumoniae strains were separated from liver abscesses and the magA gene was frequently present; however, in our study, the K. pneumoniae strains were separated from various clinical specimens and the magA gene had low frequency.



Author's Affiliation
  • Pegah Shakib

    Google Scholar Pubmed

  • Morovvat Taheri Kalani

    Google Scholar Pubmed

  • Rashid Ramazanzadeh

    Email I'd for correspondance: atrop_t51@yahoo.com
    Google Scholar Pubmed

  • Amjad Ahmadi

    Google Scholar Pubmed

  • Samaneh Rouhi

    Google Scholar Pubmed

Article Details

Issue: Vol 5 No 8 (2018)
Page No.: 2581-2589
Published: Aug 24, 2018
Section: Research articles
DOI: https://doi.org/10.15419/bmrat.v5i8.467

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Creative Commons License

Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 How to Cite
Shakib, P., Kalani, M., Ramazanzadeh, R., Ahmadi, A., & Rouhi, S. (2018). Molecular detection of virulence genes in Klebsiella Pneumoniae clinical isolates from Kurdistan Province, Iran. Biomedical Research and Therapy, 5(8), 2581-2589. https://doi.org/https://doi.org/10.15419/bmrat.v5i8.467

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