Biomedical Research and Therapy <p>Biomedical Research and Therapy (ISSN 2198-4093)<strong>&nbsp;</strong>is the major forum for basic and translational research into therapies. An international peer-reviewed journal, it publishes high quality open access research articles with a special emphasis on basic, translational and clinical research into molecular therapeutics and cellular therapies, including animal models and clinical trials. The journal also provides reviews, viewpoints, commentaries and reports.&nbsp;</p> <p>Biomedical Research and Therapy's Editorial Policies follow the recommendations of the <a href="" target="_blank" rel="noopener">International Committee of Medical Journal Editors (ICMJE)</a>, <a href="" target="_blank" rel="noopener">the World Association of Medical Editors (WAME)</a>, and&nbsp;<a href="" target="_blank" rel="noopener">the Committee on Publication Ethics (COPE)</a> for guidance on policies and procedures related to publication ethics.&nbsp;The journal is published monthly, <em><strong>12 issues</strong></em> per year.</p> BioMedPress (BMP) en-US Biomedical Research and Therapy 2198-4093 <p>Copyright The Author(s) 2017. This article is published with open access by <a href="" target="_blank">BioMedPress</a>. This article is distributed under the terms of the&nbsp;<a href="" target="_blank">Creative Commons Attribution License (CC-BY 4.0)</a> which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.&nbsp;</p> Allergic rhinitis complicated by hypertrophy of the mucous membrane of nasal turbinates in patients of Northern Vietnam <p><strong>Introduction</strong>: Constant contact with allergens contributes to the formation of allergic rhinitis and swelling of the mucous membrane of the nasal cavity. Moreover, constant inflammation stimulates hypertrophy of the mucous membrane of the nasal turbinates and paranasal sinuses. This study aimed to assess the clinical features of hypertrophic rhinitis (HR) in combination with allergic rhinitis (AR) in patients from Northern Vietnam to improve the effectiveness of treatment and quality of life of patients with this pathology of the nasal cavity.</p> <p><strong>Methods</strong>: The study was conducted from June to September 2018 by the Department of Otorhinolaryngology, Thai Nguyen Central General Hospital, Vietnam. A total of 158 patients with symptoms of chronic rhinitis, aged 5–70 years, were examined. All patients with allergic rhinitis were subjected to standard, specific allergological tests, such as the determination of specific IgE in the blood, and statistics were assessed according to the otorhinolaryngology examination and CT scan. All patients were also examined by otolaryngologists.</p> <p><strong>Results</strong>: According to the results of our study, among 64 patients with a diagnosis of AR, a total of 45 (70.31%) patients were diagnosed as AR in combination with a hypertrophy of the mucous membrane of the nasal turbinate, with an average duration of morbidity of 5.8 ±1.6 years. Moreover, 17 (37.78%) patients were diagnosed with severe hypertrophy of the mucous membrane of the nasal inferior turbinate (the nasal inferior turbinate is 50% or more of nasal cavity and with nasal polyps). The sensitization to house dust mites and fungi was high.</p> <p><strong>Conclusion</strong>: The peculiar findings of AR in combination with HR detected in patients of Northern Vietnam was associated with the onset of the disease at the age of 20-40 years, the predominance in men, the predominance of the disease in smokers, and the predominance in residents of cities and industrial areas (71.1%). Indeed, there was an observed combination of AR with severe and moderate degree of HR. The sensitization of patients with AR in combination with HR in Northern Vietnam are similar to those in Asia, with predominant allergy to house dust mites.</p> Kryukov Andrei Ivanovich Galina Petrovna Bondareva Nguyen Thi Phuong Thao ##submission.copyrightStatement## 2020-06-24 2020-06-24 7 6 3813 3818 10.15419/bmrat.v7i6.609 title description none g In vitro antimicrobial activity of hydrosol from Litsea cubeba (Lour.) Pers. against Helicobacter pylori and Candida albicans <div data-id="paragraph-e4ac0e5eb983"><strong>Introduction</strong>: <em>Helicobacter pylori</em> and<em> Candida albicans</em> are classified as the most common pathogenic agents in humans.<em> H. pylori</em> is responsible for gastroduodenal diseases and greatly associated with gastric carcinogenesis, while <em>C. albicans</em> is the main cause of fungal urinary tract, genital yeast, and fungal skin infections. The increasing appearance of drug-resistant strains of <em>H. pylori</em> and <em>C. albicans</em> has made the treatment of the infections more serious. Hydrosols from plant steam distillation have been traditionally used in medicine, cosmetics, and culinary uses. They have been recently suggested as antimicrobial agents owing to their safety and ability to reduce the potential of resistance. The aim of the present study is to assess antibacterial and antifungal activities of hydrosols extracted from the fresh fruits of <em>Litsea cubeba</em> against <em>H. pylori</em> and <em>C. albicans</em>.</div> <div data-id="paragraph-e4ac0e5eb983"><strong>Methods</strong>: The <em>L. cubeba</em> fruit hydrosol was obtained by steam distillation method. Evaluation of the growth-inhibiting and microbicidal effects of the hydrosol towards the <em>H. pylori</em> ATCC 43504 and <em>C. albicans</em> ATCC 10231 was determined through MIC (minimal inhibitory concentration), MBC (minimal bactericidal concentration), and MFC (minimal fungicidal concentration) measurements using broth dilution assays. Compositions of the dissolved essential oil (dEO) from the hydrosol were analyzed by GC-MS (gas chromatography-mass spectrometry).</div> <div data-id="paragraph-e4ac0e5eb983"><strong>Results</strong>: The results indicated that the<em> L. cubeba</em> fruit hydrosol exhibited strong antimicrobial ability towards the bacterium <em>H. pylori</em> (MIC of 10%, MBC of 30%) and the yeast <em>C. albicans</em> (MIC of 10%, MFC of 40%). The cells of <em>H. pylori</em> and<em> C. albicans</em> were killed completely after 24 and 18 hours of treatment with 30% and 40% of the hydrosol, respectively. The major constituents of the dEO were geranial (32.92%), neral (27.12%), p-menthan-8-yl acetate (8.45%), 2-cyclopropyl-2-methylspiro[2.2]pentane-1-carboxylic acid (8.09%), linalool (4.24%), and methyl heptenone (4.15%).</div> <div data-id="paragraph-e4ac0e5eb983"><strong>Conclusion</strong>: The results of the study suggest that <em>L. cubeba</em> fruit hydrosols could be used as potent natural antibacterial and antifungal preparations in the global effort to discover safe alternatives to toxic antimicrobial agents.</div> <div data-id="p-2084ca267f85">&nbsp;</div> Tran Thanh Hung Pham Thu Trang Hoang Viet Nguyen Thi My Lan Luong Thi My Ngan Tran Trung Hieu ##submission.copyrightStatement## 2020-06-25 2020-06-25 7 6 3819 3828 10.15419/bmrat.v7i6.610 title description none g Personalized correction of lipid metabolism in blood of inhabitants of the metropolis under high technogenic load <p><strong>Introduction</strong>: The purpose of the study was to evaluate the effectiveness of personalized correction of violations of fat metabolism when using an individually prescribed vitamin and mineral complex.</p> <p><strong>Methods</strong>: The study included 313 volunteers who belong to the category of &amp;quot;practically healthy people&amp;quot; and do not have a severe chronic pathology. All participants in the study were randomly assigned to the main group (n = 197) and the comparison group (n = 116). At the first stage, the state of blood lipid metabolism was evaluated in the representatives of both formed groups, and the laboratory examination complex included: determination of the total cholesterol concentration, the concentration of low- and high-density cholesterol, and the level of triglycerides. Patients of the main group were additionally monitored for the level of blood trace elements and a wide range of biochemical parameters. Taking into account the results of the latter, the composition of the vitamin and mineral complex was individually selected for them. The duration of its daily intake for all members of the main group was 60 days. Patients in the comparison group received a placebo for a similar time period.</p> <p><strong>Result &amp; Conclusion</strong>: The study of the effectiveness of the personalized vitamin and mineral complex allowed us to demonstrate the positive effect of daily intake (for 2 months) on the parameters of blood lipid metabolism. It was manifested in a decrease in the total concentration of cholesterol, which was mainly provided by a decrease in the level of cholesterol contained in low-density.</p> Andrew K. Martusevich Konstantin A. Karuzin ##submission.copyrightStatement## 2020-06-26 2020-06-26 7 6 3829 3834 10.15419/bmrat.v7i6.611 title description none g Evaluation of the role of kras gene in colon cancer pathway using string and Cytoscape software <div data-id="paragraph-f960d921e693"><strong>Introduction</strong>: cancer is one of the top three most commonly occurring cancer worldwide with more than 1.8 million cases in 2018. In Malaysia, cancer is the most common cancer in males and the second most common cancer in females. Albeit being the second most common form of cancer in Malaysia, there is a lack of informal or structured national cancer screening program in Malaysia and it remains a low priority in healthcare planning and expenditure. The risk of developing colon cancer is greatly influenced by factors such as lifestyle habits, genetic inheritance, diet, weight, and exercise. KRAS, the most frequently mutated oncogene in cancer, occurs in about 50 percent of cancers. This study maps the KRAS gene involved in colon cancer pathway using applications such as STRING version 11.0 and version 3.7.0 to provide a clear visualization of all the related and involved proteins and genes that interact with KRAS gene in the pathway.</div> <div data-id="paragraph-f960d921e693"><strong>Methods</strong>: Using KRAS as a seed, a protein-protein interaction network was constructed with 3391 interactions which were retrieved from the STRING version 11.0 database. The protein network interaction was further grouped into 6 clusters using the MCODE application. Molecular function and biological processes of the genes involved in the KRAS protein network were determined using Biological Networks Gene Ontology (BiNGO).</div> <div data-id="paragraph-f960d921e693"><strong>Results</strong>: According to the resulting protein-protein network interaction map, it revealed that KRAS mechanism and co-expressed genes interconnected with protein or enzyme binding, receptor signaling protein activity and vascular endothelial growth factor (VEGF) receptor 2 binding.</div> <div data-id="paragraph-f960d921e693"><strong>Conclusion</strong>: Understanding these protein-protein interactions provide insight into cellular activities and thus aid in the understanding of the cause of disease.</div> <div data-id="p-204db851f9a4">&nbsp;</div> Sandya Menon Prabhakaran Menon Asita Elengoe ##submission.copyrightStatement## 2020-06-27 2020-06-27 7 6 3835 3842 10.15419/bmrat.v7i6.612 title description none g Antioxidant effects of hydro-alcoholic extract of Ashrasi date palm in hepatotoxicity caused by mercuric chloride administration <p><strong>Introduction</strong>: date palm (ADP) is a flowering plant type of the palm family which has strong antioxidant properties. Mercuric chloride (MC) is a complex substance that generates oxidative stress. This study was designed to investigate the protective effects of ADP on the , inflammatory, oxidative and apoptotic changes induced by MC.</p> <p><strong>Methods</strong>: Fifty-six male rats were randomly allocated into 8 groups (n = 7) as follows: Group 1- control, 2- MC (50 mg/kg), 3-5: ADP (30, 90, and 270 mg/kg), and 6-8: MC+ADP. The rats were orally and administrated with the respective agents for 5 weeks. Extracts of ADP were prepared and screening was conducted. Nitric oxide (NO), lipid (LP), and Ferric Reducing Ability of Plasma (FRAP) assays were conducted to assess the antioxidant index. The levels of p53, Bcl2 and Bax gene expression were assessed by real-time PCR. Cytokines involved in inflammation were evaluated by enzyme-linked assay (ELISA). Liver enzymes were also measured using biochemical methods. changes (e.g. in hepatocyte (HD) and central hepatic vein (CHV)), and apoptosis cell index were evaluated by light and fluorescent microscopy methods.</p> <p><strong>Results</strong>: MC treatment significantly increased all parameters described in the Methods section (except for FRAP level and Bcl2 expression level, which were both decreased) in the MC group, when compared to the control group (P &amp;lt; 0.05). Also, all indices were significantly decreased in the ADP and ADP+MC groups (except for FRAP level and Bcl2 expression level, which were increased), when compared to the MC group (P &amp;lt; 0.05).</p> <p><strong>Conclusion</strong>: These findings revealed that ADP extract can serve as a potent antioxidant which effectively attenuates the adverse effects of MC on the liver through the activation of antioxidant mechanisms and regeneration of modifications.</p> Shiva Roshankhah Ahmad Shabanizadeh Amir Abdolmaleki Mohammad Reza Salahshoor ##submission.copyrightStatement## 2020-06-28 2020-06-28 7 6 3843 3854 10.15419/bmrat.v7i6.613 title description none g